Increased monoamine oxidase activity and imidazoline binding sites in insulin-resistant adipocytes from obese Zucker rats

Figure 7 Saturation binding analysis with ³H-2-(2-benzofuranyl)-2-imidazoline and ³H-idazoxan in adipocyte membranes from white subcutaneous adipose tissue of lean and obese rats. A and B: Adipocyte membranes from subcutaneous white adipose tissue of lean (open circles) or obese (dark circles) littermates were incubated for 45 min with increasing concentrations of ³H-2-(2-benzofuranyl)-2-imidazoline alone (A) or ³H-idazoxan plus 10 µmol/L rauwolscine (B). For each tested concentration, total binding was measured in the absence of any competitor while non-specific binding was determined in the presence of 100 µmol/L cirazoline. Left panels show the saturation curves of specific binding, with each point corresponding to the mean ± SEM of three separate saturation experiments. Right panels show the Scatchard plot (bound/free vs bound) of the saturation experiment for each radioligand. Calculated parameters of saturation experiments are reported in Table 2.