Increased monoamine oxidase activity and imidazoline binding sites in insulin-resistant adipocytes from obese Zucker rats

Figure 2 Insulin-like effects of vanadium combined with hydrogen peroxide or with amine oxidase substrates on hexose uptake into rat adipocytes. Sodium orthovanadate was present at 100 µmol/L in all the conditions: Alone (control) or combined with 100 nmol/L insulin, or with 1 mmol/L hydrogen peroxide, tyramine, and benzylamine. The amines were tested without and with 1 mmol/L phenelzine. 2-deoxyglucose (2-DG) uptake is expressed as nmoles of intracellular 2-DG/100 mg lipids/5 min. Mean ± SEM of five lean (open columns) and five obese male rats (shaded columns). Significantly different from corresponding condition in lean at: ^aP < 0.05; ^bP < 0.01. Blockade of tyramine (tyr) or benzylamine (benz) effect by phenelzine was significant at P < 0.001 in both genotypes.