Regeneration and DNA demethylation do not trigger PDX-1 expression in rat hepatocytes

Figure 2 Quantitative real time polymerase chain reaction amplification profiles of GAPDH (green line), Hxk 2 (red line) and PDX-1 (blue line), and relative melting curves from total RNA of Clone-9 hepatocytes grown with or without 5 μmol/L 5-aza-deoxycytidine for 4 d. In the graphic representation below, data from at least three independent experiments that used different RNA preparations were normalized vs the negative control (mean ± SD); the Hxk 2 mRNA levels increase when cells are cultured in the presence of the drug, as expected, but no increase in the PDX-1 mRNA was observed. 5-aza-dC: 5-aza-deoxycytidine.