Regeneration and DNA demethylation do not trigger PDX-1 expression in rat hepatocytes

Figure 1 Schematic representation of the timing and molecular events that occur during liver regeneration. A: Schematic representation of the timing and molecular events that occur during liver regeneration. In the right panel, proliferating cell nuclear antigen immunodetection confirmed that, 24 h after hepatectomy, cell proliferation was reactivated; B: Real time polymerase chain reaction (RT-PCR) amplification of some important genes involved in insulin synthesis from pancreas and regenerating liver total RNA; C: RT-PCR amplification of the PDX-1 gene from pancreas and liver total RNA. PC-1: Pro-hormone convertase 1; Ins-1: Insulin 1; Ngn3: Neurogenin 3; Btc: β-cellulin.