Protective effects of anti-ricin A-chain antibodies delivered intracellularly against ricin-induced cytotoxicity

Figure 5 Intracellular localization of fluorescent-labeled ricin and MTS-conjugated dgA Ab by immunofluorescent staining. A: Immunofluorescent staining of RAW264.7 cells treated with Alexa 488-labeled ricin (green); B: MTS-conjugated dgA Ab detected by TRITC-labeled anti-mouse IgG (red); C: Superposition of A and B demonstrated co-localization of ricin and MTS-dgA Ab (yellow); D: Immunofluorescent staining of RAW264.7 cells treated with unconjugated dgA Ab and detected with TRITC-labeled anti-mouse IgG. No staining was observed, demonstrating the inability of unconjugated dgA Ab to enter cells.