Protective effects of anti-ricin A-chain antibodies delivered intracellularly against ricin-induced cytotoxicity

Figure 2 Intracellular localization of fluorescent-labeled ricin and fluorescent-labeled DelIN-encapsulated RAC18 mAb by immunofluorescent staining. Immunofluorescent staining of RAW264.7 cells treated with Alexa 488-labeled ricin and DelIN-encapsulated Alexa 594-labeled RAC18 mAb. A: Alexa 488-labeled ricin (green); B: DelIN-Alexa 594-labeled RAC18 mAb (red); C: Superposition of A and B demonstrated co-localization of ricin and RAC18 mAb (yellow); D: Immunofluorescent staining of RAW264.7 cells treated with naked Alexa 594-labeled RAC18 mAb. No staining was observed, demonstrating the inability of naked RAC18 mAb to enter cells.