Discovery of the strong antioxidant selenoneine in tuna and selenium redox metabolism

Figure 2 Effect of selenoneine on cell growth. A: Control; B: Selenoneine. Human umbilical vein endothelial cells (HUVECs) were cultured by supplementation with 10% calf serum in the presence of selenoneine (50 μmol/L) or its absence in MCDB131 medium at 37°C for 24 h. Cell extracts with distilled water were subjected to liquid chromatography inductively coupled plasma mass spectroscopy (LC-ICP-MS) by monitoring ⁸²Se. The arrow shows a peak of selenoneine detected in the HUVEC extract with distilled water at a retention time of 10.5 min. Thus, selenoneine was incorporated into the cells and promoted cell growth.