Soluble and insoluble signals sculpt osteogenesis in angiogenesis

Figure 4 Calvarial tissue regeneration by doses of gamma-irradiated recombinant hOP-1 implanted in non-healing calvarial defects of non-human primates P. ursinus. A: Heterotopic ossicle generated 30 d upon implantation of 0.5 mg hOP-1 in the rectus abdominis of P. ursinus showing prominent osteoid seams in orange red (long arrows) surfacing mineralized newly formed bone in blue (inset right panel of A); B-E: Tissue induction and morphogenesis by gamma-irradiated hOP-1 osteogenic devices implanted in calvarial defects and harvested on day 15 (B: 0.1 mg hOP-1), 30 (C, D: 0.5 mg hOP-1) and 90 (E: 0.5 mg hOP-1) after implantation, respectively. Note the time course of osteogenic tissue forming between the osteogenic pericranial and endocranial fronts with restitutio ad integrum 90 d after implantation; F, G: Solid cords of mineralized remodeled newly formed bone upon implantation of 2.5 mg gamma-irradiated hOP-1 in non-healing calvarial defects of P. ursinus. A-G: Undecalcified sections cut at 6 μm stained free-floating with a modified Goldner’s trichrome.