Localization and function of a eukaryotic-initiation-factor-2-associated 67-kDa glycoprotein

Figure 2 p67 is a ribosome-associated protein. pETFVA^-/p67-transfected cells were labeled with 35S-Met/Cys and lysed with 0.5 mL ribosome binding solution with 0.5% NP-40. The cell extracts were loaded on 4 mL of a 10%-40% linear sucrose gradient. The samples were centrifuged at 150 000 g for 2 h and fractionated into nine portions. A: 20 mL of each fraction was resolved on SDS-PAGE and autoradiographed (right panel). The intensity of p67 was quantitated using NIH image (left panel); B: Optical densities of each fraction were measured at 260 nm.