Localization and function of a eukaryotic-initiation-factor-2-associated 67-kDa glycoprotein

Figure 1 Overexpressed p67 in the cytosol. COS-1 cells were transfected with a p67 expression vector, fixed with 4% paraformaldehyde, and permeabilized with 0.1% saponin. The cells were incubated with mouse anti-p67 monoclonal antibody or anti-mouse IgG polyclonal antibody. After washing with PBS, the cells were incubated with fluorescein-conjugated goat anti-mouse antibody. The localization of p67 was visualized by fluorescence microscopy.