Single-nucleus RNA sequencing and spatial transcriptomics reveal the mechanism by which Xiaozhiling injection treats internal hemorrhoids

Figure 3 Spatial transcriptome. A: Hematoxylin and eosin-stained section 1 week after Xiaozhiling injection; B: Spatial transcriptome; C: Differences in expression of the classic fibroblast marker genes collagen 3 alpha 1, decorin, and actin alpha 2 in different clusters; D: Spatial localization of the classic fibroblast marker genes collagen 3 alpha 1, decorin, and actin alpha 2; E: Spatial localization of sphingosine kinase 1, sphingosine-1-phosphate receptor 1, and transforming growth factor-β1; F: Differences in expression of sphingosine kinase 1, sphingosine-1-phosphate receptor 1, and transforming growth factor-β1 in different clusters; G: Analysis of differences between the two groups. COL3A1: Collagen 3 alpha 1; DCN: Decorin; ACTA2: Actin alpha 2; Sphk1: Sphingosine kinase 1; S1PR1: Sphingosine-1-phosphate receptor 1; TGF-β1: Transforming growth factor-β1.