Single-nucleus RNA sequencing and spatial transcriptomics reveal the mechanism by which Xiaozhiling injection treats internal hemorrhoids

Figure 1 Single-cell transcriptome map. A: Uniform manifold approximation and projection (UMAP) of cell types, with different colors representing different cell types; B: Visualization of cell dimension reduction in the modeling group and Xiaozhiling group, with different colors representing different groups; C: Bubble map of classic marker genes in each cell type; D: Cell proportion diagrams for the model group and Xiaozhiling group, with different colors representing different clusters; E: Analysis of large groups of differentially expressed genes in fibroblasts; F: UMAP of fibroblasts; G: UMAP of Xiaozhiling-treated fibroblasts; H: Proportions of fibroblasts in different groups; I: Volcano plot of differentially expressed genes (actin alpha 2, myosin heavy chain 1, myosin light chain kinase, and transgelin) in fibroblasts; J: Violin plot of classic fibroblast markers in each cluster; K: UMAP of classic fibroblast markers. UMAP: Uniform manifold approximation and projection; NK: Natural killer; TAGLN: Transgelin; ACTA2: Actin alpha 2; MYH11: Myosin heavy chain 1, MYLK: Myosin light chain kinase; CK: Control group.