Extracellular matrix gene set and microRNA network in intestinal ischemia-reperfusion injury: Insights from RNA sequencing for diagnosis and therapy

Table 1 RNA sequencing exploration of RNA-regulated extracellular matrix gene sets

Gene	miRNA regulation	miRNA regulation
COL1A2	miR-29 family (miR-29a/b/c) targets COL1A2 mRNA, inhibiting its expression	COL1A2 mRNA stability and translation are regulated by RNA-binding proteins such as HuR and TIA-1/TIAR
THY1	miR-21 and miR-199a suppress THY1 expression	THY1 mRNA expression is regulated by various extracellular matrix factors (e.g., TGF-β and FGF-2) and their receptors
IL10	miR-106b and miR-155 reduce IL10 expression by targeting IL10 mRNA	IL10 expression is regulated by inflammation-related signaling pathways (e.g., NF-κB and STAT3)
MMP2	miR-21, miR-221/222, and other miRNAs regulate MMP2 expression by targeting MMP2 mRNA	MMP2 expression is regulated by various extracellular matrix molecules (e.g., ECM components) and their receptors
SERPINH1	miR-29 family (miR-29a/b/c) targets SERPINH1 mRNA, inhibiting its expression	SERPINH1 expression is regulated by extracellular matrix components (e.g., collagen) and related signaling pathways
COL3A1	miR-29 family (miR-29a/b/c) regulates COL3A1 expression by targeting its mRNA	COL3A1 expression is regulated by extracellular matrix components and related signaling pathways
COL14A1	miR-29 family (miR-29a/b/c) affects COL14A1 expression by targeting its mRNA	COL14A1 expression is regulated by extracellular matrix components and related signaling pathways
P4HA1	miR-124, miR-29 family (miR-29a/b/c), and other miRNAs regulate P4HA1 mRNA expression levels	P4HA1 expression is regulated by oxygen concentration and extracellular matrix environment (e.g., collagen content)

miRNA: MicroRNA; MMP2: Matrix metalloproteinase 2; ECM: Extracellular matrix.