Zinc pretreatment for protection against intestinal ischemia-reperfusion injury

Figure 5 Zinc protects small intestinal organoids from hypoxia-reoxygenation injury. A and B: Small intestinal organoids were cultured for five days, followed by the addition of ZnSO₄. After 24 or 48 hours of treatment, the organoids were subjected to 2 hours of hypoxia followed by reoxygenation. Representative images of the organoids are shown; C: Quantification of dead organoids is presented. Scale bar, 200 mm; All the data are presented as the means ± SEM. ^aP < 0.05.