Zinc pretreatment for protection against intestinal ischemia-reperfusion injury

Figure 4 Zinc reduces reactive oxygen species production in Caco-2 cells. A: Caco-2 cells were cultured under hypoxic conditions for 12 hours. After reoxygenation for 3 hours, superoxide dismutase levels were measured; B and C: Caco-2 cells were incubated with DCFH-DA under hypoxia-reoxygenation conditions, and DCF fluorescence was measured to evaluate intracellular oxidative stress. All the data are presented as the means ± SEM. ^aP < 0.01; ^bP < 0.0001. SOD: Superoxide dismutase.