Zinc pretreatment for protection against intestinal ischemia-reperfusion injury

Figure 3 Zinc pretreatment reduces the expression of inflammatory cytokines in tissues in the abdominal aortic occlusion model. mRNA levels of Il1β, Il6, and TNFα were measured using a qPCR assay in gut, liver, and kidney tissues, respectively. The ischemia-reperfusion (I/R) group and the I/R + ZnSO₄ group underwent I/R procedures, while the Sham group underwent only abdominal opening. ZnSO₄ was administered via intraperitoneal injection at a concentration of 10 mg/kg. All the data are presented as the means ± SEM. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001, ^dP < 0.0001. I/R: Ischemia-reperfusion.