Zinc pretreatment for protection against intestinal ischemia-reperfusion injury

Figure 2 Zinc pretreatment mitigates multi-organ damage in abdominal aortic occlusion model. A: Hematoxylin and eosin (HE) staining of intestinal tissue sections and Chiu’s intestinal injury score. Scale bar, 50 mm; B: HE staining of liver tissue sections and the Suzuki liver Injury score. Scale bar, 20 mm; C: Periodic acid-Schiff staining of kidney tissue sections and renal tubular injury score. Scale bar, 20 mm; D: Serum alanine aminotransferase levels; E: Serum aspartate aminotransferase levels; F: Serum creatinine levels; G: Serum urea levels. The ischemia-reperfusion (I/R) group and the I/R + ZnSO₄ group underwent I/R procedures, while the Sham group underwent only abdominal opening. ZnSO₄ was administered via intraperitoneal injection at a concentration of 10 mg/kg. All the data are presented as the means ± SEM. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001, ^dP < 0.0001. HE: Hematoxylin and eosin; AST: Aspartate aminotransferase; ALT: Alanine aminotransferase; Scr: Serum creatinine; I/R: Ischemia-reperfusion. AAO: Abdominal aortic occlusion.