Ligamentum teres hepatis as a graft for portal and/or superior mesenteric vein reconstruction: From bench to bedside

Figure 4 Distribution and function of endothelial cells and relative content of collagen fibers, elastic fibers and smooth muscle in ligamentum teres hepatis. A: The ligamentum teres hepatis (LTH) lumen (× 100 magnification); B: Endothelial cells (black arrow, × 400 magnification); C: LTH after Verhoeff-Van Gieson (VVG) staining (× 400 magnification); D: The portal vein (PV) after VVG staining. Elastic fibers (red), collagen fibers (black) and smooth muscle (yellow) (× 400 magnification); E: CD34 expression in LTH endothelial cells (× 400 magnification); F: CD34 expression in PV endothelial cells (× 400 magnification); G: Factor VIII-related antigen (FVIIIAg) expression in LTH endothelial cells (× 400 magnification); H: FVIIIAg expression in PV endothelial cells (× 400 magnification); I: Endothelial nitric oxide synthase (eNOS) expression in LTH endothelial cells (× 400 magnification); J: eNOS expression in PV endothelial cells (× 400 magnification); K: Tissue type plasminogen activator (t-PA) expression in LTH endothelial cells (× 400 magnification); L: Tissue type plasminogen activator expression in PV endothelial cells (× 400 magnification).