Hydrogen gas and preservation of intestinal stem cells in mesenteric ischemia and reperfusion

Figure 5 Mucosal oxidative injury in the ischemia-reperfusion model. A: Immunostaining for 8-hydroxy-2'-deoxyguanosine showed limited oxidative stress at the mucosa in the ischemia group, although there was considerable pyknosis at the tip of the villi; B: Extensive oxidative injury throughout the epithelium, including the crypt base, in the reperfusion group; C: Mild-to-moderate oxidative injury at the mucosa of the crypt base, along with pyknosis with the denudation at the tip of the villi, in the hydrogen group. Scale bar: 50 μm.