Hydrogen gas and preservation of intestinal stem cells in mesenteric ischemia and reperfusion

Figure 2 Intestinal stem cell injury by ischemia-reperfusion injury. A: Immunofluorescence staining with caspase-3 (green) revealed mucosal injury at the epithelial layer closed to the tip of the villi in the ischemia group; B: Extensive apoptosis was identified at the crypt base and the whole epithelial layer in the reperfusion group; C: Apoptosis at the crypt base was limited in the hydrogen group. Scale bar: 100 μm.