Novel roles of lipopolysaccharide and TLR4/NF-κB signaling pathway in inflammatory response to liver injury in Budd-Chiari syndrome

Table 1 Primers used in this study

	Forward	Reverse
β-actin (150 bp)	5’-CCCATCTATGAGGGTTACGC-3’	5’-TTTAATGTCACGCACGATTTC-3’
TLR4 (186 bp)	5’-GCCGGAAAGTTATTGTGGTGGT-3’	5’-ATGGGTTTTAGGCGCAGAGTTT-3’
NF-κB p65 (108 bp)	5’-AAGATCTGCCGAGTAAACCG-3	5’-TCCCGTGAAATACACCTCAA-3’
IL-2 (113 bp)	5’-CAAGCAGGCCACAGAATTGA-3’	5’-TTCCAGCGTCTTCCAAGTGA-3’
TNF-α (89 bp)	5’-AGGAGGGAGAACAGCAACTC-3’	5’-TGTATGAGAGGGACGGAACC-3’
IFN-γ (130 bp)	5’-CAGGCCATCAGCAACAACAT-3’	5’-GCTGGATCTGTGGGTTGTTC-3’

TLR4: Toll-like receptor 4; NF-κB: Nuclear factor-κB; TNF-α: Tumor necrosis factor-α; IL-2: Interleukin-2; IFN-γ: Interferon-γ.