Basic Study
Copyright ©The Author(s) 2022.
World J Diabetes. Aug 15, 2022; 13(8): 622-642
Published online Aug 15, 2022. doi: 10.4239/wjd.v13.i8.622
Table 1 Statistical analysis of wound healing rate at different times
Time
0 d (%)
3 d (%)
7 d (%)
14 d (%)
Control group010.41 ± 0.2417.43 ± 0.1333.73 ± 0.28
Experimental group032.78 ± 0.4638.14 ± 0.2853.11 ± 0.07
Homogeneity of variance
P value< 0.05< 0.05< 0.05
Table 2 Gas chromatography–mass spectrometry full spectrum identification results
No.
RT (min)
Relative content (%)
Qualitative
CAS
Name
126.360.5951.56512-61-8(-)-α-Santalene
227.070.2625.8187-44-5Caryophyllene
329.711.8665.77495-60-3α-Zingiberene
429.830.6414.58495-61-46-methyl-2-(4-methylcyclohex-3-enyl)hept-1,5-diene
530.641.9560.8520307-83-9β-Sesquiphellandrene
630.71.3380.1644-30-4α-Curcumene
734.210.3826.4756192-70-2(Z)-a-Atlantone
834.270.2361.921139-30-6Caryophyllene oxide
934.360.3019.5283173-76-62-Methyl-1-(4-methylphenyl)-3-buten-1-ol
1034.520.2558.19108549-47-9(6Z)-2-Methyl-6-(4-methyl-3-cyclohexen-1-ylidene)-2-hepten-4-one
1135.470.5829.36639-99-6Elemol
1235.780.2858.71145512-84-1Trans-Sesquisabinene hydrate
13360.3762.0258334-55-7Zingiberenol
1436.570.7486.36989-21-5Atractylon
1536.830.2512.582508-14-32-methyl-6-(4-methylidenecyclohex-2-en-1-yl)hept-2-en-4-one
1637.230.7722.651209-71-8γ-Eudesmol
1737.4120.7491.81180315-67-7Tumerone
1837.6713.8022.041460-73-7Agarospirol
1937.811.7538112-39-0Methyl palmitate
2038.411.2641.2473-16-5a-Eudesmol
2138.480.729.97515-20-8Costol
2238.659.0676.48473-15-4β-Eudesmol
2339.124.3397.75532-65-0ar-Turmerone
2439.280.2411.6719888-00-74,8-Cycloundecadien-1-ol, 6,6,9-trimethyl-2-methylene-, (1R,4E,8E)-
2539.470.234.4265646-68-6N-(4-hydroxyphenyl)retinamide
2639.640.398.2219912-67-5(+)-Epicubenol
2739.90.8549.4730666-87-62-Methyl-6-(4-methylphenyl)-4-heptanone
2840.161.0549.0772441-71-5(6R,7R)-Bisabolone
2940.532.2389.2108645-54-1(E)-Atlantone
3040.890.6582.79120681-80-32-Cyclohexene-1-propanol,g-methyl-4-methylene-a-(2-methyl-1-propen-1-yl)
3141.580.4575.15112-61-8Octadecanoic acid, methyl ester
3241.881.8716.03112-62-9Methyl oleate
3341.980.657.881937-62-8trans-octadec-9-enoicacidmethylester
3442.551.0326.03112-63-0methyl linoleate
3543.540.4242.85301-00-89,12,15-Octadecatrienoic acid, methyl ester
3643.610.224.6329550-55-8Teresantalol(7CI)
3744.580.2488.569301-27-52-(1,5-Dimethyl-4-Hexenyl)-5-Methyl-Phenol
3843.850.846.2338142-57-32-Methyl-6-(p-tolyl)hept-2-en-4-ol
3945.011.1128.143218-36-84-Biphenylcarboxaldehyde
4046.860.8475.644666-84-6Proximadiol
4147.750.4698.07949081-10-1(S)-3-Methyl-6-((S)-6-methyl-4-oxohept-5-en-2-yl)cyclohex-2-enone
4248.710.249.542566-90-7Methyl 4,7,10,13,16,19-cis-docosahexanenoate
4351.082.31%83.121957-10-3Palmitic acid
Table 3 Ultra-performance liquid chromatography full spectrum identification results
No.
Name
CAS       
Molecular weight
RT (min)
Relative concentration (μg/mL)
1DL-Arginine7200-25-1174.111750.78324.44253696
2Nitrosobis(2-oxopropyl)amine60599-38-4158.069140.81715.74503993
3Gluconic acid526-95-4196.057650.82182.48616363
4D-(+)-Proline344-25-2115.063570.84615.35704499
5Cabotegravir1051375-10-0405.111820.84720.08010135
6α,α-Trehalose99-20-7342.116230.847409.0449144
7D-(-)-Quinic acid77-95-2192.062750.8556.2670819
8Isocitric acid320-77-4192.026370.93963.29259087
9Citric acid77-92-9192.026371.169145.2856943
10Gallic acid149-91-7170.020781.89923.57871448
11Chlorogenic acid327-97-9354.095275.33244.58986172
12Catechin88191-48-4290.079175.43198.07463825
13methyl chlorogenate123483-19-2368.110815.50118.15074983
146-Acetylcodeine6703-27-1341.162375.88612.3548527
152-(3,4-Dihydroxyphenyl)ethyl 3-O-(6-deoxy-β-L-mannopyranosyl)-6-O-[(2E)-3-(3,4-dihydroxyphenyl)-2-propenoyl]-β-D-glucopyranoside61303-13-7624.206176.09220.78761602
16(3R,5R)-1,3,5-Trihydroxy-4-{[(2E)-3-(4-hydroxy-3-methoxyphenyl)-2-propenoyl]oxy}cyclohexanecarboxylic acid2613-86-7368.110846.196201.3429867
17Emodin-8-Beta-D-Glucoside23313-21-5432.105716.5797.280885459
182-[4-[3-[3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyphenyl]-7-hydroxy-2,3-dihydrochromen-4-one74639-14-8550.169026.62513.98565399
19Baicalin21967-41-9446.08526.66316.85569046
20Liquiritin551-15-5418.126766.73722.91985259
21Naringin10236-47-2580.179646.95926.36327029
22Hesperidin520-26-3610.190387.19185.82076776
23Azelaic acid123-99-9188.10427.5484.773123466
24Berberine2086-83-1335.115258.242232.2489146
25isosakuranetin-7-O-rutinoside14259-47-3594.195568.4934.34803523
26Tinnevellin glucoside80358-06-1408.142288.78613.59498227
27Daidzein486-66-8254.057789.00913.05981793
28Chrysophanol 8-O-β-D-glucoside13241-28-6416.110999.0117.88080565
29Licoricesaponin G2118441-84-2838.399989.8247.195669531
30Luteolin491-70-3286.047769.9091.034661042
31(15Z)-9,12,13-Trihydroxy-15-octadecenoic acid95341-44-9330.240719.93819.41925634
32Rheic acid478-43-3284.0321111.02981.8044913
33Bisdemethoxycurcumin24939-16-0308.1049611.328153.9247824
346-Hydroxy-2-naphthoic acid16712-64-4188.0465211.3287.047135466
35Demethoxycurcumin22608-11-3338.1156711.473151.9906609
365,7-dihydroxy-2-(4-hydroxy-3-methoxyphenyl)-6-(3-methylbut-2-enyl)-2,3-dihydrochromen-4-one76735-58-5370.1416811.54155.08087646
37Curcumin458-37-7368.1260611.619307.1972754
38Isoimperatorin482-45-1270.0891811.8471.025466018
39Genistein446-72-0270.0527912.256153.8276373
40(+/-)9-HODE98524-19-7296.2351113.14623.43829567
41(+)-ar-Turmerone532-65-0216.1513513.60523.84749189
42Indane496-11-7118.0784313.60527.63249372
43Prespatane100387-71-8204.1877713.9562.010509748
44(+)-Nootkatone4674-50-4218.1669614.49716.6606139
45Aristolone6831-17-0218.1669614.58845.88543598
462,2'-Methylenebis(4-methyl-6-tert-butylphenol)119-47-1340.2401516.78825.70628216
Table 4 Ruyi Jinhuang powder “Chinese medicine-ingredients-target” correspondence
CAS
Name
GenecardID
Attribution
512-61-8(-)-α-SantalenePTGS2, CHRM2JH
87-44-5CaryophyllenePTGS2, CHRM2BZ, JH, CZ, ZHP, TNX
495-60-3α-ZingiberenePTGS2, DPP4JH
20307-83-9β-SesquiphellandrenePTGS2JH
644-30-4α-CurcumenePTGS2, TOP2A, DPP4JH
1139-30-6Caryophyllene oxideCHRM2, DPP4ZHP, TNX
639-99-6ElemolCHRM2ZHP, JH
58334-55-7ZingiberenolCHRM2JH
6989-21-5AtractylonSCN5A, CHRM4, HTR2A, CHRM2, OPRM1CZ
82508-14-32-methyl-6-(4-methylidenecyclohex-2-en-1-yl)hept-2-en-4-onePTGS2, CHRM2JH, ZHP, CZ
112-39-0Methyl palmitatePTGS2, IL-10, TNF, IL-6JH, ZHP, BZ, HB, THF
473-15-4beta-EudesmolCHRM2ZHP, JH, CZ
532-65-0(6S)-2-methyl-6-(4-methylphenyl)hept-2-en-4-oneDPP4, PTGS2, CHRM2x, ampCJH
112-62-9Methyl oleateRXRAZHP, THF
1937-62-8trans-octadec-9-enoicacidmethylesterRXRAZHP, THF
112-63-0methyl linoleatePTGS2, RXRABZ, THF, ZHP, TNX
301-00-89,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z)-PTGS2, RXRATHF
38142-57-32-Methyl-6-(p-tolyl)hept-2-en-4-olPTGS2, CHRM2, DPP4, ampC, RXRATHF, ZHP, TNX
1957—10—3Palmitic acidPTGS2, IL-10, TNF, PCYT1ADH, ZHP, THF
149-91-7Gallic acidPTGS2, PTPN1, TOP2ADH, TNX, CP, GC, JH
23313-21-5Emodin-8-Beta-D-GlucosideTOP2ADH
458-37-7CurcuminPTGS2, PTPN1JH
22608-11-3DemethoxycurcuminPTGS2, PTPN1, AKR1B1, ABCB1, SCN5AJH
520-26-3HesperidinPTGS2CP
14259-47-3isosakuranetin-7-O-rutinosideTOP2ACP
10236-47-2NaringinTOP2A, CDKN1A, TNF, RASGRF2, RAF, PTGS2, ampC, MTTP, APOB, mvaA, PPARACP, GC
21967-41-9BaicalinPTPN1, TOP2ADH
88191-48-4CatechinCHRM2DH, CP
491-70-3LuteolinPTGS2, DPP4, EGFR, IL10, CDK4, TNF, IL6, NFKBIA, psdA1, IL2, TOP2A, SLC2A4, INSR, METCP, ZHP
551-15-5LiquiritinPTGS2GC
478-43-3Rheic acidPTGS2, AKR1B1DH
2086-83-1BerberinePTGS2, RXRAHB
77-92-9Citric acidPTGS2, AKR1B1, GRIN2A, GRIA2, PTPN1DH
320-77-4Isocitric acidPTGS2DH
7200-25-1DL-ArgininePTGS2THF
446-72-0GenisteinPTGS2, EGFR, TNF, SELE, IL8GC
482-45-1IsoimperatorinPTGS2BZ
486-66-8DaidzeinPTGS2, RXRAGC
6831-17-0AristoloneCHRM2, PTGS2BZ
532-65-0(+) -ar-TurmeronePTGS2, RXRA, CHRM2, ampC, DPP4JH
327-97-9Chlorogenic acidPTGS2HB
77-95-2D-(-)-Quinic acidPTGS2HB
1460-73-7AgarospirolCHRM2ZHP
Table 5 Docking parameters of chemical components and target protein molecules
Name
Target
LibDockScore
LuteolinTOP2A104.333
Methyl palmitatePTGS2114.221
ar-TurmericPTGS291.0625
CHRM288.3765
Methyl LinoleateRXRA138.583
PTGS2130.838
Palmitic acidPTGS2114.349
DemethoxycurcuminPTGS2135.479
NaringinTOP2A141.197
PTGS2164.305