Basic Study
Copyright ©The Author(s) 2019.
World J Diabetes. Jul 15, 2019; 10(7): 396-402
Published online Jul 15, 2019. doi: 10.4239/wjd.v10.i7.396
Table 1 Polymerase chain reaction primers details
GenePrimersBase pairsPCR cycleAmplicon size
Epidermal growth factor receptor (EGFR) rs17337023Forward outer: ATTAACCACCAATCCAACATCCAGAC2667 °C; 30 s; 30 cyclesT allele 180; C allele 271; Control 406
Reverse outer: CTTTCCCTCCACTGAGGACAAAGTT25
Forward inner (A allele): TCTCTTTCACTTCCTACAGATGCTCA26
Reverse inner (T allele): AGCCTTCAAGACCTGGCGCA20
Table 2 Descriptive statistics and genotype frequency of study subjects
Hypertensive pregnant case (n = 122)Normotensive pregnant control (n = 80)P-value
Age (Yr)30.55 ± 8.0529.13 ± 10.190.054
Weight (kg)77.56 ± 16.8869.24 ± 11.070.025
Body Fat %35.138 ± 4.2925.01 ± 8.280.000
Waist circumference (cm)104.50 ± 12.0986.92 ± 12.030.000
Systolic blood pressure (mmHg)131.76 ± 13.04122.02 ± 8.270.000
Diastolic blood pressure (mmHg)85.88 ± 8.4573.31 ± 11.270.000
Walk
None90.1%17.7%0.000
30 min/3 days week7.9%74.2%
30 min/5 days week2.0%8.1%
Genotype frequency
EGFR rs17337023 polymorphism
AA30190.079
AT5648
TT3613
Allele Odds Ratio
Allele A1.282 [0.860-1.912]0.219
Allele T0.780 [0.523-1.163]0.221