Short-term effects of obestatin on hexose uptake and triacylglycerol breakdown in human subcutaneous adipocytes

Table 1 Clinical parameters of the study group and characteristics of adipocyte preparations

Clinical characteristics of SCAT donors
BMI of subjects, kg/m2	26.1 ± 0.7
Age, yr	40 ± 3
Biochemical features of adipocyte preparations
Cell lipid content/lipolysis assay, mg (n)	14.1 ± 1.3 (7)
Cell lipid content/glucose uptake assay, mg (n)	15.9 ± 1.3 (10)
Lipolytic responsiveness (fold increase over basal glycerol release, n = 7)
Basal	1.00 ± 0.17
Isoprenaline 10 μmol/L	5.14 ± 0.67b
Human atrial natriuretic peptide 1 μmol/L	5.16 ± 0.44b
Glucose transport capacity (fold increase over basal 2DG uptake, n = 10)
Basal	1.00 ± 0.13
Insulin 100 nmol/L	3.14 ± 0.28b
Hydrogen peroxide 1 mmol/L	1.72 ± 0.27a

Adipocytes were isolated by liberase digestion from pieces of SCAT obtained from a total of 13 women then incubated for lipolysis and/or glucose uptake assays for the number of individual preparations indicated in parenthesis. Different from respective basal values at:

^aP < 0.05;

^bP < 0.001. SCAT: Subcutaneous adipose tissue.