Effect of medicinal mushrooms on blood cells under conditions of diabetes mellitus

Figure 2 Immunocytochemical analysis of peripheral blood leukocytes in rats depending on the content of p53 pro-apoptotic protein in healthy animals, animals with streptozotocin-induced diabetes and treated with submerged cultured mycelium powder of mushrooms. A: Control; B: Control animals treated with Agaricus brasiliensis (A. brasiliensis); С: Animals with STZ-induced diabetes mellitus; D: Diabetic animals treated with A. brasiliensis. 1: p53^-; 2: p53⁺; 3: p53⁺⁺. An indirect immunoperoxidase method was used for detection and visualization of intracellular protein p53. The content analysis of p53 in leukocytes of rat peripheral blood was performed by light microscopy using a × 40 microscope objective. Depending on intensity of staining, the cells were divided into 3 groups: Negative reaction (p53^-), positive reaction (p53⁺), and high-positive (p53⁺⁺) reaction.