Preliminary study on overproduction of reactive oxygen species by neutrophils in diabetes mellitus

Figure 3 Total reactive oxygen species produced by neutrophils of non-diabetic and diabetic subjects. Neutrophils isolated from non-diabetic individuals and diabetic patients subjected for ROS secretion at resting condition and upon stimulation with PMA. The maximal production of ROS at any time point over a period of 50 s was recorded and compared. Neutrophils from diabetic patients showed a higher production of ROS at resting and activated conditions. Data expressed as mean ± SD and the statistical significance was determined at ^aP value ≤ 0.05. ROS: Reactive oxygen species; PMA: Phorbol myristate acetate.