Preliminary study on overproduction of reactive oxygen species by neutrophils in diabetes mellitus

Figure 2 Temporal analysis of reactive oxygen species secreted by neutrophils of non-diabetic and diabetic subjects. Isolated neutrophils were measured for ROS secretion via chemiluminescence method at every 10 s over a period of 50 s. Neutrophils isolated from diabetic patients showed a higher and consistent secretion of ROS in resting condition (A). PMA stimulation elevated the ROS secretion from neutrophils of both non-diabetic and diabetic subjects. Neutrophils from diabetic patients produced a higher amount of ROS compared to non-diabetic subjects. ROS production was determined at every 10 s beginning from the administration of PMA to the time point where the ROS production reached a maximal level and started to decline (B). Data expressed as mean ± SD and the statistical significance was determined at ^bP value ≤ 0.001. ROS: Reactive oxygen species; PMA: Phorbol myristate acetate.