Transcriptional factors, Mafs and their biological roles

Figure 4 Comparison between histological changes and the Oil-Red-O staining of stop-mafA-siRNA- and mafA-siRNA-treated cells. Mouse 3T3-L1 pre-adipocytes were induced to differentiate, and Mafa SiRNA was transfected using a transfection reagent. The morphological appearances of the pre-adipocyte culture before induction and 7 d after induction were then compared. The morphology of the 3T3-L1 cells was directly observed, and lipid droplets were stained using Oil Red O. Oil Red O staining was compared between untreated and Mafa-siRNA-treated cells. Intracellular lipid staining was not observed in the Mafa-siRNA-treated cells.