Roles of interstitial fluid pH in diabetes mellitus: Glycolysis and mitochondrial function

Figure 3 Insulin binding to insulin receptor under various pH conditions. After serum starved for 4 h, L6 myotubes were treated with 100 nmol/L insulin for 15 min in the HEPES buffer with different values of pH. Proteins expressed on the plasma membrane were biotinylated and precipitated. Differentiated L6 myotubes were treated with [¹²⁵I]-labeled insulin for 15 min in the indicated pH buffers, and the radioactivities were measured after cells were washed and suspended. The values of radioactivity from at least 6 experiments are shown. The values are shown as the mean ± SEM. ^aP < 0.05, ^bP < 0.01 vs pH 7.4. Modified from ref.[46] with allowance of non-profit use of figures.