Copyright
©2014 Baishideng Publishing Group Co.
World J Diabetes. Feb 15, 2014; 5(1): 59-68
Published online Feb 15, 2014. doi: 10.4239/wjd.v5.i1.59
Published online Feb 15, 2014. doi: 10.4239/wjd.v5.i1.59
Figure 5 Immunofluorescence staining of differentiated PDX1-monomeric red fluorescent protein infected islets with primary PDX1 and insulin antibodies with secondary antibodies conjugated to Alexa-Fluor488 (green).
PDX1+ cells (RFP) nuclei stain positive with PDX1/Alexa-488 antibody confirming lentiviral expression. Insulin/Alexa-Fluor488 (green) stains insulin within PDX1+ infected cells (yellow). Nuclei are stained blue with DAPI. Scale bars are 20 μm. RFP: Red fluorescent protein.
- Citation: Seeberger KL, Anderson SJ, Ellis CE, Yeung TY, Korbutt GS. Identification and differentiation of PDX1 β-cell progenitors within the human pancreatic epithelium. World J Diabetes 2014; 5(1): 59-68
- URL: https://www.wjgnet.com/1948-9358/full/v5/i1/59.htm
- DOI: https://dx.doi.org/10.4239/wjd.v5.i1.59