Nkx6.2 synergizes with Cdx-2 in stimulating proglucagon gene expression

Figure 4 Effect of ectopic Nkx6. 2 expression on endogenous Gcg expression in the In111 and IEC6 cells. A: Transient transfection of Cdx-2, Nkx6.1 and Nkx6.2 in In111 cells. Cell lysates from In111 cells transfected with Myc-taged Cdx-2, Nkx6.1 and Nkx6.2 was prepared and analyzed for their expression by Western blotting. The same membrane was stripped and re-hybridized against the β-actin antibody; B: The expression of endogenous Gcg mRNA was assayed by real time RT-PCR. The Gcg mRNA expression was quantified against that of actin; C: Transient transfection of Nkx6.2 (by anti-Myc tag antibody) and Cdx-2 (by anti-Cdx-2 antibody) in the IEC6 cell line; D: The expression of Gcg mRNA was assayed by real time RT-PCR.