Nkx6.2 synergizes with Cdx-2 in stimulating proglucagon gene expression

Figure 3 The interaction between Cdx-2 and Nkx6. 2. A: Purification of GST and GST-Nkx6.2 fusion protein from bacteria lysate. Lane 1: GST; Lane 2: GST-Nkx6.2; M: molecular weight marker; B1: Left panel. GST-Cdx-2 pulls down Nkx6.2 but not Nkx6.1. GST-Cdx-2 column was incubated with lysate either from pcDNA3-Nkx6.1-myc transfected BHK cells (lanes 1, 2 and 3) or from pcDNA3-Nkx6.2-myc transfected cells (lanes 4, 5 and 6). Myc tag antibody was utilized in Western blotting; B2: Right panel. GST-Nkx6.2 pulls down Cdx-2. GST-Nkx6.2 column was incubated with cell lysate from pcDNA3-Cdx-2-myc transfected BHK cells.