Aucubin mitigates the elevation of microglial aerobic glycolysis and inflammation in diabetic neuropathic pain via aldose reductase

Figure 6 Aucubin fails to restore aerobic glycolysis and inflammation in the context of AKR1B1 deficiency. A: The expression level of AKR1B1 after transfecting AKR1B1 shRNA lentivirus. The quantitative analysis of the relative expression of AKR1B1 below. Data are expressed as mean ± SEM (n = 4); B: The mRNA levels of pro-inflammatory cytokine; C-F: The experimental program of extracellular acidification rate (C and D) and the oxygen consumption rate (E and F) of BV-2 measured by Seahorse XFe96 Extracellular Flux Analyzer. Data are expressed as mean ± SEM (n = 6). ^aP < 0.01, ^bP < 0.05, high glucose vs high glucose + aucubin. NS: No significance; Auc: Aucubin; 2-DG: 2-deoxy-d-glucose; ECAR: Extracellular acidification rate; OCR: Oxygen consumption rate.