TERT/FOXO1 signaling promotes islet β-cell dysfunction in type 2 diabetes mellitus by regulating ATG9A-mediated autophagy

Figure 4 TERT activates FOXO1 transcription to inhibit ATG9A expression. A: JASPAR was used to analyze the binding of FOXO1 to the ATG9A promoter; B and C: Dual-luciferase reporter gene and ChIP assays were used to verify the targeting of FOXO1 to ATG9A; D and E: Reverse transcription quantitative polymerase chain reaction and western blotting were used to analyze the expression of TERT, FOXO1 and ATG9A in cells after the cells were transfected with sh-TERT or/and sh-ATG9A. The data are expressed as mean ± SD. Each experiment was conducted in triplicate. ^aP < 0.05 vs sh-NC, IgG, and NC groups, ^bP < 0.05 vs sh-NC, IgG, and NC groups, ^cP < 0.05 vs sh-TERT + sh-NC group. HG: High glucose.