TERT/FOXO1 signaling promotes islet β-cell dysfunction in type 2 diabetes mellitus by regulating ATG9A-mediated autophagy

Figure 3 FOXO1 knockdown suppresses type 2 diabetes mellitus progression in mice. sh-FOXO1 was injected into type 2 diabetes mellitus mice. A and B: FOXO1 mRNA and protein expression was analyzed via reverse transcription quantitative polymerase chain reaction and western blot; C and D: Body weight and fasting blood glucose; E: Insulin levels were measured via ELISAs; F: Morphology of islet tissues via H&E staining (× 200); G: Insulin levels were analyzed via immunohistochemistry (× 200); H: Western blotting was used to assess the expression of LC3B and p62. The data are expressed as means ± SD. n = 10 for in vivo experiments. ^aP < 0.05 vs STZ/HFD + sh-NC group, ^bP < 0.01 vs STZ/HFD + sh-NC group. FBG: Fasting blood glucose; HFD: High-fat diet; STZ: Streptozotocin.