Neurophysiological mechanisms of electroacupuncture in regulating pancreatic function and adipose tissue expansion

Figure 5 Modulation of pancreatic function by TRPV1 neuronal pathways in electroacupuncture-treated t10 dorsal root ganglion. A: Representative immunofluorescence images illustrating co-expression of insulin (INS) and CGRP in the pancreas at 200 × magnification. Nuclei are stained blue with 4',6-diamidino-2-phenylindole, with red representing INS and green representing CGRP immunofluorescence; B-D: Electrophysiological activity measurements of the T10 DRG in model mice under different conditions: Untreated model, TRPV1 knockout (TRPV1^-/-) in T10 DRG, and TRPV1^-/- in T10 DRG followed by electroacupuncture (EA) at the ST25 acupoint; E: Discharge frequency associated with the groups mentioned in B-D; F-H: Impact of EA on the expression levels of TRPV1 and INS in the pancreas following capsazepine injection into the T10 DRG. GAPDH served as an internal reference protein. ^aP < 0.05 compared to the model or another treatment group. INS: Insulin; DAPI: 4',6-diamidino-2-phenylindole; ST25: Tianshu acupoint.