Curcumol ameliorates diabetic retinopathy via modulating fat mass and obesity-associated protein-demethylated MAF transcription factor G antisense RNA 1

Figure 6 Curcumol exerts its protective effects via the fat mass and obesity-associated/MAF transcription factor G antisense RNA 1 axis. A and B: Fat mass and obesity-associated protein (FTO) expression in high glucose-human retinal vascular endothelial cells models (curcumol, curcumol + si-FTO, curcumol + si-FTO + MAF transcription factor G antisense RNA 1) detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot assays; C: MAF transcription factor G antisense RNA 1 expression in cell models measured by qRT-PCR; D: Methyl tetrazolium assay to measure cell proliferation; E and F: Cell colony assay to detect cell viability; G and H: Transwell migration assay to evaluate cell migration; I and J: Levels of lactate dehydrogenase and malondialdehyde, both indicative of HG-induced cellular injury, detected after treatment with curcumol; K-M: qRT-PCR analysis of high glucose-induced transcription of inflammatory cytokines upon curcumol treatment. Data represent the mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. HG-HRVECs: High glucose-human retinal vascular endothelial cells; FTO: Fat mass and obesity-associated protein; MAFG-AS1: MAF transcription factor G antisense RNA 1; LDH: Lactate dehydrogenase; MDA: Malondialdehyde; TNF: Tumor necrosis factor; IL: Interleukin.