Curcumol ameliorates diabetic retinopathy via modulating fat mass and obesity-associated protein-demethylated MAF transcription factor G antisense RNA 1

Figure 5 MAF transcription factor G antisense RNA 1 expression is demethylated by fat mass and obesity-associated in human retinal vascular endothelial cells. A: Predicted m6A modification sites on MAF transcription factor G antisense RNA 1 (MAFG-AS1) using the SRAMP tool; B: Expression of MAFG-AS1 after knockdown of common m6A writers; C: Fat mass and obesity-associated protein (FTO) knockdown or overexpression in human retinal vascular endothelial cells (HRVECs) confirmed by Western blot; D: MAFG-AS1 expression in FTO overexpressed HRVECs was measured by quantitative real-time polymerase chain reaction (qRT-PCR); E: MAFG-AS1 expression in cycloleucine-treated HRVECs analyzed by qRT-PCR; F: Methylated RNA immunoprecipitation-qPCR analysis of the m6A modification level of MAFG-AS1 in HRVECs after FTO knockdown; G: qRT-PCR analysis of FTO expression in high glucose (HG)-HRVECs and HG-HRVECs treated with curcumol; H: qRT-PCR analysis of FTO expression in HRVECs and HG-HRVECs; I and J: FTO expression in the retinal tissue of control mice, type 1 diabetes mice, and type 1 diabetes mice treated with curcumol detected by qRT-PCR and Western blot. Data represent the mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, NS: No significance. HRVECs: Human retinal vascular endothelial cells; MAFG-AS1: MAF transcription factor G antisense RNA 1; METTL: Methyltransferase-like; WTAP: Wilms’ tumor 1-associating protein; FTO: Fat mass and obesity-associated protein; ALKBH5: Anaplastic lymphoma kinase B homolog 5; HG: High glucose; MeRIP: Methylated RNA immunoprecipitation; T1D: Type 1 diabetes.