Curcumol ameliorates diabetic retinopathy via modulating fat mass and obesity-associated protein-demethylated MAF transcription factor G antisense RNA 1

Figure 4 MAF transcription factor G antisense RNA 1 mediates the inhibitory effects of curcumol on high glucose-induced human retinal vascular endothelial cells. A: MAF transcription factor G antisense RNA 1 expression in high glucose (HG)-human retinal vascular endothelial cells models (control, curcumol, and curcumol + si-MAF transcription factor G antisense RNA 1) was measured by quantitative real-time polymerase chain reaction; B: Methyl tetrazolium assay to measure cell proliferation; C and D: Cell colony assay to detect cell viability; E and F: Transwell migration assay to evaluate cell migration; G and H: Levels of lactate dehydrogenase and malondialdehyde, both indicative of HG-induced cellular injury, were detected upon treatment with curcumol; I-K: The expression of HG-induced transcription of inflammatory cytokines upon curcumol treatment was detected by quantitative real-time polymerase chain reaction. Data represent the mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. HG-HRVECs: High glucose-human retinal vascular endothelial cells; MAFG-AS1: MAF transcription factor G antisense RNA 1; LDH: Lactate dehydrogenase; MDA: Malondialdehyde; TNF: Tumor necrosis factor; IL: Interleukin.