Role and mechanism of Roux-en-Y gastric bypass in the treatment of diabetic urinary bladder hyperactivity by reducing TRPV1 and P2X3

Figure 4 Morphological analysis results of mouse bladder. A: Representative images of bladder hematoxylin and eosin (H&E) staining; B: The thickness of bladder epithelial layer; C: Representative images of Masson’s trichrome staining of the bladder; D: The ratio of collagen fibers in the bladder muscle layer; E: Western blot representative image of bladder tissue alpha-smooth muscle actin (α-SMA); F: Statistical results of bladder α-SMA (n = 8). ^aP < 0.05, overactive bladder (OAB) group compared with the sham group; ^bP < 0.05, Roux-en-Y gastric bypass (RYGB) group compared with the OAB group. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.