Dl-3-n-butylphthalide ameliorates diabetic foot ulcer by inhibiting apoptosis and promoting angiogenesis

Figure 5 Dl-3-n-butylphthalide reduces cell apoptosis under high glucose conditions. A: Flow cytometry detection of cell apoptosis of Human umbilical vein endothelial cells; B: Flow cytometry detection of human keratinocytes cells; C: Flow cytometry detection of human dermal fibroblasts; D: Statistical results of apoptotic cells in flow cytometry; E: TUNEL staining was detected the cell apoptosis of human umbilical vein endothelial cells; F: TUNEL staining of human keratinocytes cells; G: TUNEL staining of human dermal fibroblasts; H: Statistical analysis of the percentage of apoptotic cells according to the result of the TUNEL assay; I: Apoptosis-related protein expression was examined by Western blot analysis. Data expressed as individual values with mean ± SD. ^dP < 0.0001, ^cP < 0.001, ^bP < 0.01, ^aP < 0.05. HG: High glucose; NBP: Dl-3-n-butylphthalide; HUVEC: Human umbilical vein endothelial cells; HaCaT: Human keratinocyte cells; HDF: Human dermal fibroblasts.