MicroRNA-122-5p is upregulated in diabetic foot ulcers and decelerates the transition from the inflammatory to the proliferative stage

Figure 5 Adeno-associated virus-DJ-microRNA-122-5p up-decelerated the transition from the inflammatory to the proliferative stage. A: In situ hybridization was performed using a microRNA (miR)-122-5p-specific probe. Green indicates miR-122-5p expression, and blue indicates 4’,6-diamidino-2-phenylindole (20 ×); B: Tissue immunofuorescence staining of F4/80, ARG1, and inducible nitric oxide synthase (20 ×); C and D: The migration capacity and quantitative analysis of NIH3T3 cultured with RAW264.7 media in different groups were assessed using a cell scratch assay (n = 3); E and F: Matrix metalloproteinase 9, vascular endothelial growth factor, inflammation factors, and fibrosis factors were detected in vivo by enzyme-linked immunosorbent assay assays (n = 3). ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. miR: MicroRNA; DU: Diabetic ulcer; AAVDJ: Adeno-associated virus-DJ; DAPI: 4’,6-diamidino-2-phenylindole; iNOS: Inducible nitric oxide synthase; TNF-α: Tumor necrosis factor-α; HIF-1α: Hypoxia inducible factor-1α; MMP9: Matrix metalloproteinase 9; SMA: Smooth muscle actin; VEGF: Vascular endothelial growth factor; FN: Fibronectin; NC: Normal control.