MicroRNA-122-5p is upregulated in diabetic foot ulcers and decelerates the transition from the inflammatory to the proliferative stage

Figure 3 MicroRNA-122-5p upregulated matrix metalloproteinase 9 to target inflammatory factors during the transition from the inflammatory phase to the proliferative phase. A and B: Levels of matrix metalloproteinase (MMP) 9, tumor necrosis factor (TNF)-α, and hypoxia inducible factor (HIF)-1α in wound tissues of mice after 14 days were detected using immunohistochemistry (20 ×); C: The expression of MMP9, TNF-α, and HIF-1α in wound tissues of mice was assessed using quantitative real-time polymerase chain reaction; D and E: Expressions of MMP9, TNF-α, and HIF-1αproteins were tested in wound tissues (n = 3); F and G: Expressions of MMP9, TNF-α, and HIF-1α proteins were tested in NIH3T3 cells in different groups (n = 3). ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. DU: Diabetic ulcer; miR: MicroRNA; AAVDJ: Adeno-associated virus-DJ; TNF-α: Tumor necrosis factor-α; HIF-1α: Hypoxia inducible factor-1α; MMP9: Matrix metalloproteinase 9; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; NC: Normal control.