NR4A1 silencing alleviates high-glucose-stimulated HK-2 cells pyroptosis and fibrosis via hindering NLRP3 activation and PI3K/AKT pathway

Figure 6 Silencing of nuclear receptor subfamily 4 group A member 1 attenuated phosphoinositide 3-kinase/protein kinase B pathway-mediated fibrosis in high glucose-activated HK-2 cells. A: Illustrative Western blotting bands and integrated density analysis of protein kinase B (AKT), p-AKT, phosphoinositide 3-kinase (PI3K), and p-PI3K in HK-2 cells; B: Representative western blotting and integrated density analysis of transforming growth factor-β1 in HK-2 cells; C: Illustrative immunocytochemistry images of collagen III in HK-2 cells. Scale bar = 20 μm, n = 3. ^bP < 0.05 vs normal control, ^cP < 0.05 vs high glucose + negative control. HG: High glucose; NC: Normal control; NR4A1: Nuclear receptor subfamily 4 group A member 1; AKT: Protein kinase B; PI3K: Phosphoinositide 3-kinase; si-NR4A1: SiRNA targeting nuclear receptor subfamily 4 group A member 1; TGF-β1: Transforming growth factor-β1.