NR4A1 silencing alleviates high-glucose-stimulated HK-2 cells pyroptosis and fibrosis via hindering NLRP3 activation and PI3K/AKT pathway

Figure 3 Markers of NOD-like receptor protein 3-mediated pyroptosis were increased in rats with diabetic kidney disease. A: Representative western blotting bands and integrated density analysis of NOD-like receptor protein 3, cleaved-caspase-1, and gasdermin D N-terminal structural domain in rat kidney tissues; B: Quantitative real-time PCR of interleukin (IL)-1β mRNA expression in rat kidney tissues and concentration of IL-1β in rat serum, n = 3. ^aP < 0.05 vs control. CON: Control; DKD: Diabetic kidney disease; GSDMD-N: Gasdermin D N-terminal structural domain; IL-1β: Interleukin-1β; NLRP3: NOD-like receptor protein 3.