O-linked β-N-acetylglucosamine transferase regulates macrophage polarization in diabetic periodontitis: In vivo and in vitro study

Figure 8 O-GlcNAc transferase inhibitor suppresses M1 polarization and facilitates M2 polarization. A: After O-GlcNAc transferase inhibitor (OSMI4) treatment, O-linked β-N-acetylglucosamine levels were measured using Western blot; B: mRNA expression of inducible nitric oxide synthase and interleukin (IL)-6; C: IL-10 and arginase 1 in cells was measured using quantitative real-time polymerase chain reaction; D: Cluster of differentiation (CD) 86; E: CD206 levels were observed using immunofluorescence staining. Scale bar: 50 μm; F: Relative fluorescent intensity of CD86 and CD206 was quantified; G and H: CD86 and CD206 levels were evaluated and quantified using flow cytometry. ^aP < 0.05. ¹P vs control. ²P vs lipopolysaccharide + high glucose + dimethyl sulfoxide. LPS: Lipopolysaccharide; HG: High glucose; sh-NC: Short hairpin RNA-negative control; sh-OGT: Short hairpin RNA targeting O-GlcNAc transferase; iNOS: Inducible nitric oxide synthase; IL: Interleukin; CD: Cluster of differentiation; Arg1: Arginase 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; MSO: Dimethyl sulfoxide; OSMI4: O-GlcNAc transferase inhibitor; DAPI: 4’,6-Diamidino-2-phenylindole.