O-linked β-N-acetylglucosamine transferase regulates macrophage polarization in diabetic periodontitis: In vivo and in vitro study

Figure 7 Silencing of O-GlcNAc transferase inhibits M1 polarization and facilitates M2 polarization. A: mRNA expression of inducible nitric oxide synthase and interleukin (IL)-6; B: IL-10 and arginase 1 in cells was measured using quantitative real-time polymerase chain reaction; C: Immunofluorescence staining was conducted to measure cluster of differentiation (CD) 86; D: CD206 levels. Scale bar: 50 μm; E: Relative fluorescence intensity of CD86 and CD206 was quantified; F and G: CD86 and CD206 levels were assessed and quantified by flow cytometry. ^aP < 0.05. ¹P vs control. ²P vs lipopolysaccharide + high glucose + short hairpin RNA-negative control. LPS: Lipopolysaccharide; HG: High glucose; sh-NC: Short hairpin RNA-negative control; sh-OGT: Short hairpin RNA targeting O-GlcNAc transferase; iNOS: Inducible nitric oxide synthase; IL: Interleukin; CD: Cluster of differentiation; Arg1: Arginase 1; DAPI: 4’,6-Diamidino-2-phenylindole.