LEF1 influences diabetic retinopathy and retinal pigment epithelial cell ferroptosis via the miR-495-3p/GRP78 axis through lnc-MGC

Figure 9 Animal experiments to verify that LEF1 combined with lnc-MGC regulates diabetic retinopathy progression. A and B: Diabetic retinopathy rat body weight and blood glucose detection; C: A TUNEL assay was used to detect apoptosis. The scale bar represents 100 μm; D: ELISA was used to detect the expression of the inflammatory factors TNF-α, IL-1β and IL-6; E: Reactive oxygen species were detected by flow cytometry; F-H: Western blotting was used to detect the expression of related proteins; I: HE staining was used to observe the retinal structure. The scale bar represents 20 μm. ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001. ONL: Outer nuclear layer; INL: Inner core layer; RGC: retinal ganglion cells; NC: Normal control; DR: Diabetic retinopathy; ROS: Reactive oxygen species.