LEF1 influences diabetic retinopathy and retinal pigment epithelial cell ferroptosis via the miR-495-3p/GRP78 axis through lnc-MGC

Figure 8 LEF1, as the binding protein of lnc-MGC, regulates the progression of diabetic retinopathy. A and B: LEF1 can bind to the lnc-MGC promoter; C: Western blotting was used to detect the inhibition efficiency of LEF1; D: RT-qPCR was used to detect the transfection efficiency; E: CCK-8 was used to measure the cell proliferation activity; F: TUNEL was used to detect cell apoptosis. The scale bar represents 100 μm; G: ELISA was used to determine the expression of the inflammatory cytokines TNF-α, IL-1β and IL-6; H: Flow cytometry was used to detect reactive oxygen species; I-K: Western blotting was used to detect the expression of related proteins. ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001. NC: Normal control; HG: High glucose; ROS: Reactive oxygen species.