LEF1 influences diabetic retinopathy and retinal pigment epithelial cell ferroptosis via the miR-495-3p/GRP78 axis through lnc-MGC

Figure 7 miR-495-3p regulates endoplasmic reticulum stress and ferroptosis through GRP78. A: RT-qPCR was used to detect transfection efficiency; B: Western blot was used to detect transfection efficiency; C: CCK-8 was used to detect cell proliferation activity; D: A TUNEL assay was used to detect apoptosis. The scale bar represents 100 μm; E: The expression of TNF-α, IL-1β and IL-6 was detected by ELISA; F: Reactive oxygen species were detected by flow cytometry; G-I: Western blotting was used to detect the expression of related proteins. ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001. NC: Normal control; HG: High glucose; ROS: Reactive oxygen species; mimic: miR-495-3p mimic.